Neural crest-derived mesenchymal cells support thymic reconstitution after lethal irradiation.

Abstract The thymus is composed of thymocytes and stromal cells including thymic epithelial mesenchymal cells. Thymic are originated from Neural Crest (NC) the mesoderm. Although it known that NC-derived (NCd) contribute to organogenesis development, their specific role in T cell regeneration unclear. To evaluate regenerative ability NCd thymus, we used lethally irradiated mice examine effect ionizing irradiation (IR) on size, (thymic (TECs) cells). Additionally, ablated via vitro diphtheria toxin (DT) administration double transgenic (Wnt1-Cre/+; Rosa YFP/DTRmice) express NC-specific Cre Cre-driven DT receptor (DTR) transplanted depleted mesenchyme under renal capsules non-irradiated C57BL/6 (CD45.1) mice. IR reduced size absolute numbers percentages thymocytes, primarily (CD4 +CD8 +) TECs. Moreover, number but not upregulated several hematopoietic factors such as Flt3l, Periostin, Scf, Bmp4after supported reconstitution. Taken together, our findings indicate only radio-resistant, also produce molecules may support early T-cell development for reconstitution after .